Abstract
A bacterial strain, designated BzDS03 was isolated from water sample, collected from Dal Lake Srinagar. The strain was characterized by using 16S ribosomal RNA gene and 16S-23S rRNA internal transcribed spacer region sequences. Phylogenetic analysis showed that 16S rRNA sequence of the isolate formed a monophyletic clade with genera Escherichia. The closest phylogenetic relative was Escherichia coli with 99% 16S rRNA gene sequence similarity. The result of Ribosomal database project's classifier tool revealed that the strain BzDS03 belongs to genera Escherichia.16S rRNA sequence of isolate was deposited in GenBank with accession number FJ961336. Further analysis of 16S-23S rRNA sequence of isolate confirms that the identified strain BzDS03 be assigned as the type strain of Escherichia coli with 98% 16S-23S rRNA sequence similarity. The GenBank accession number allotted for 16S-23S rRNA intergenic spacer sequence of isolate is FJ961337.
Highlights
Access to clean drinking water is key factor underpinning public healths even in areas of the world where significant investments are made to protect water quality [1]
In the present research communication, we report the genotypic characterization of strain BzDS03 on the basis of 16S rRNA gene sequence and 16S-23S rRNA internal transcribed spacer region analysis
The BLAST result showed that the 16S rDNA sequence of isolate BzDS03 has 99% sequence similarity having highest score i.e. 2137 bits with Escherichia coli DH1 (ME8569)
Summary
Access to clean drinking water is key factor underpinning public healths even in areas of the world where significant investments are made to protect water quality [1]. In the present research communication, we report the genotypic characterization of strain BzDS03 on the basis of 16S rRNA gene sequence and 16S-23S rRNA internal transcribed spacer region analysis. This kind of genotypic technique involves the amplification of any phylogenetically informative targets such as 16S rRNA gene for genera identification [2] and 16S-23S rRNA for species identification [3]. One of the hundreds of strains of the bacterium Escherichia coli, O157:H7 is an emerging cause of food-borne and waterborne illness. The presence of Escherichia coli in water sample is implicit evidence for fecal contamination and represents a threat to human and environmental health [6]. In this study we describe the isolation and identification of an unknown bacterium from the Dal
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