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Abstract
BackgroundUterine disorders are common postpartum diseases in dairy cows. In practice, uterine treatment is often based on systemic or locally applied antimicrobials with no previous identification of pathogens. Accurate on-farm diagnostics are not available, and routine testing is time-consuming and cost intensive. An accurate method that could simplify the identification of uterine pathogenic bacteria and improve pathogen-specific treatments could be an important advance to practitioners. The objective of the present study was to evaluate whether a database built with uterine bacteria from European dairy cows could be used to identify bacteria from Argentinean cows by Fourier transformed infrared (FTIR) spectroscopy. Uterine samples from 64 multiparous dairy cows with different types of vaginal discharge (VD) were collected between 5 and 60 days postpartum, analyzed by routine bacteriological testing methods and then re-evaluated by FTIR spectroscopy (n = 27).ResultsFTIR spectroscopy identified Escherichia coli in 12 out of 14 samples and Trueperella pyogenes in 8 out of 10 samples. The agreement between the two methods was good with a Kappa coefficient of 0.73. In addition, the likelihood for bacterial growth of common uterine pathogens such as E. coli and T. pyogenes tended to increase with VD score. The odds for a positive result to E. coli or T. pyogenes was 1.88 times higher in cows with fetid VD than in herdmates with clear normal VD.ConclusionsWe conclude that the presence of E. coli and T. pyogenes in uterine samples from Argentinean dairy cows can be detected with FTIR with the use of a database built with uterine bacteria from European dairy cows. Future studies are needed to determine if FTIR can be used as an alternative to routine bacteriological testing methods.
Highlights
Uterine disorders are common postpartum diseases in dairy cows
25 samples were tested by Fourier transformed infrared (FTIR): 14 were positive for E. coli, 10 for T. pyogenes and 1 sample for coagulase-negative staphylococci (CNS)
We found that FTIR spectroscopy had a good agreement with routine bacteriological testing methods for identification of well-known uterine pathogens such as E. coli and T. pyogenes
Summary
Uterine disorders are common postpartum diseases in dairy cows. In practice, uterine treatment is often based on systemic or locally applied antimicrobials with no previous identification of pathogens. Uterine samples from 64 multiparous dairy cows with different types of vaginal discharge (VD) were collected between 5 and 60 days postpartum, analyzed by routine bacteriological testing methods and re-evaluated by FTIR spectroscopy (n = 27). Metritis (MET), clinical endometritis (CE) and subclinical endometritis (SE) are common uterine disorders in dairy cows associated with lower reproductive performance and higher culling rates [1,2,3]. Risk factors for these uterine diseases mainly are problems around parturition and subsequent negative energy balance [4,5,6]. The present study focused on the wellknown E. coli and T. pyogenes pathogens
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