Abstract
Identification of Enzymes and Toxins in Venoms of Indian Cobra and Russell's Viper after Starch Gel Electrophoresis
Highlights
We have studied the properties of venoms of Indian snakes, especially Indian cobra (Baja Izaja) and Russell’s viper (Vipera russelli)
Cobra venom separated into nine components, four migrating to the positive side, four migrating to the negative side, and one remaining at the center
During electrophoresis of cobra venom, there occurred a peculiar sharp line at the position indicated by the arrow in Fig. 4 (Strip C) which was visible even when the starch block was not stained for proteins
Summary
0.4 ml of fluid was obtained from each segment by this method, an amount quite sufficient for testing for enzymes and toxicity These experiments were repeated several times to confirm the identity of different components. Phosphodiesterase, Monoesterase, and 5’-Nucleotidase-Phosphodiesterase activity was detected by the method of Sinsheimer and Koerncr [23] with calcium dinitrophenyl phosphate as the substrate It was further confirmed by covering the starch block with 0.1 y0 solution of p-nitrophenyl-UMP and incubating it at 25” in a water-saturated atmosphere. 5’-Nucleotidasc activity was detected by incubating 0.4 ml of the fluid from each segment with AMP as the substrate and estimation of inorganic phosphate was carried out by the method of Fiske and SubbaRow [24]. This experiment was repeated several times to confirm the position of the toxic components
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