Abstract

The Vitek Auto Microbic System (AMS) automated identification device was used in rapid identification of staphylococci and coliforms originating from food factory environments. From a total of 3324 isolates, an average of 76% were identified with a confidence > 50% (staphylococci: 61%, coliforms: 82%). AMS identifications of presumed staphylococci did not match well (24%) with ATB 32 STAPH identifications, but coliforms matched for 80% of the isolates compared with the API 20 E system. The differences observed between the AMS and ATB 32 STAPH in identifications could be due to inadequate isolation media, insufficient isolate purification, incorrect pre-screening of isolates or an incompetent AMS database. For coliforms, the overall performance proved sufficient for rapid screening of isolates from food factory bacterial ecology studies.

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