Abstract
BackgroundAs a tumor-accelerating transcriptional factor, E2F transcription factor 7 (E2F7) was up-regulated in many forms of cancers. Nevertheless, little has been reported about the impacts of E2F7 on oral squamous cell carcinoma (OSCC). Here, we aimed to probe whether E2F7 had influences on OSCC and its potential mechanism.MethodsThe expression of E2F7 in OSCC tissues was analyzed using the data acquired from TCGA and ONCOMINE databases. E2F7 prognostic value in OSCC patients was analyzed utilizing TCGA database. The expression of E2F7 in OSCC cell lines was detected by qRT-PCR. Gain-and loss-function of E2F7 assays in TCA-83 and CAL27 cells were performed respectively to inquire the function of E2F7. Western blotting was applied to test the alternations of EMT-related markers.ResultsIn OSCC tissues, E2F7 was highly expressed. Besides, high expression of E2F7 predicted worse prognosis in OSCC patients. Moreover, E2F7 was over-expressed in TCA-83, HSC-4 and CAL27 (all OSCC cell lines) cells relative to that in HNOK (a normal cell line) cells. Gain-and loss-function assays displayed that deficiency of E2F7 suppresses CAL27 cell growth, migration, invasion and E2F7 high-expression resulted in inverse outcomes in TCA-83 cells. Finally, we found that silencing of E2F7 facilitated E-cadherin protein expression level and reduced N-cadherin, Vimentin and Snail protein levels in CAL27 cells, whilst E2F7 high-expression exhibited the opposite effects in TCA-83 cells.ConclusionsThese outcomes indicated that E2F7 performs a carcinogenic role in OSCC, which provides a theoretical basis for the therapeutic strategies of OSCC.
Highlights
Oral squamous cell carcinoma (OSCC) represents as one of the most prevalent cause of cancer death around the world, especially in males (M:F = 10.8:1) [1, 2]
E2F transcription factor 7 (E2F7) is up-regulated in oral squamous cell carcinoma (OSCC) tissues and associated with the prognosis of OSCC patients To examine whether E2F7 was correlated with OSCC development, we primarily analyzed E2F7 expression in OSCC tissues by utilizing RNA-Seq data from TCGA
Over-expression and deficiency of E2F7 in OSCC cells we explored the expression of E2F7 in OSCC cell lines
Summary
Oral squamous cell carcinoma (OSCC) represents as one of the most prevalent cause of cancer death around the world, especially in males (M:F = 10.8:1) [1, 2]. E2F transcription factors exerted effects in regulating cell angiogenesis, apoptosis and cell cycle in cancers [8]. Given the momentous effects of E2F2 in cancers, Li et al [10] suggested that E2F2 polymorphisms could be utilized to predict the risk of oropharynx squamous cell carcinoma recurrence. There are few reports about the impacts of E2Fs on OSCC, and the related mechanism has not been clarified, and needs further elaboration. Little has been reported about the impacts of E2F7 on oral squamous cell carcinoma (OSCC). We aimed to probe whether E2F7 had influences on OSCC and its potential mechanism
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