Identification of dsRNAs Associated with Soybean Dwarf Virus-Infected Soybean

Abstract

Double-stranded (ds) RNA was isolated from leaves of soybean cultivar Wayne infected with the dwarfing (D) or yellowing (Y) strain of soybean dwarf virus (SDV). Each strain produced two virus-specific dsRNAs. SDC-D dsRNAs were estimated to have molecular weights of 3.4 and 1.9×10 6 Da. The corresponding SDV-Y species were larger, having estimated molecular weights of 3.6 and 2.2×10 6 Da. Northern blot hybridization analyses showed the strains are related and that the two dsRNAs correspond to viral genomic-length and 3' subgenomic-length species (...)