Identification of driver gene alterations in Chinese female patients with squamous cell carcinomas of the lung.

Abstract

e19087 Background: Smoking remains the leading risk factor of NSCLC. Activating mutations in EGFR gene have been reported more frequently in never smokers than smokers with NSCLC. Female pts with squamous cell carcinomas of the lung (SQCLC) differ from males in that most females are life-long never/light smokers. However, molecular abnormalities in female pts with SQCLC have not been well-characterized. Methods: We retrospectively screened 38 female and 40 male pts with SQCLC in China from 2009 to 2012 to determine the prevalence of alterations in EGFR, KRAS, ALK, PI3K, PTEN and FGFR1. Mutational analysis of EGFR, PIK3CA, KRAS and PTEN was performed using PCR-based DNA sequencing. FGFR1 amplification and ALKrearrangements were detected by FISH. A Cox regression model was used to assess the association between genomic mutation status and clinical features as well as OS. Results: 10.3% (8/78) pts were detected with EGFR mutation, 6.4% (5/78) with KRAS mutation, 7.7% (6/78) with PI3K mutation and 6.4% (5/78)...