Identification of Dominant Bacteria from Crowded Places of Vellore by using MIC and ABST Analysis

Abstract

The purpose of this study is to understand the antimicrobial effect of spices and leaf extracts against prominent bacteria present in crowded localities. It has been shown that spices offer therapeutic benefits, particularly antimicrobial action. In addition to being food preservatives, they are employed as household medications. In this work, agar well diffusion method is used to compare the susceptibility of a few human pathogenic bacteria to different spice extracts. The extracts were prepared using 70% ethyl alcohol. The samples for the pathogenic bacteria were collected from crowded places situated in Vellore, Tamil Nadu. The antimicrobial properties of bilva, lemon leaves, mehndi, cinnamon, and hibiscus leaves were found to be comparatively higher than those of the other spices and leaf extracts examined. Of all the spices, ethanol extracts from cinnamon had the strongest antimicrobial properties. It has been discovered that Gram negative bacteria are more susceptible to leaf extracts and spices than Gram positive bacteria. The potential application of spices as antimicrobial agents is quite high. Key Words: spices, leaf extracts, antimicrobial activity, ethanol extract, MIC, ABST