Abstract
A limited number of reports have investigated the role of microRNAs in osteosarcoma. In this study, we performed miRNA expression profiling of osteosarcoma cell lines, tumor samples, and normal human osteoblasts. Twenty-two differentially expressed microRNAs were identified using high throughput real-time PCR analysis, and 4 (miR-135b, miR-150, miR-542-5p, and miR-652) were confirmed and validated in a different group of tumors. Both miR-135b and miR-150 have been previously shown to be important in cancer. We hypothesize that dysregulation of differentially expressed microRNAs may contribute to tumorigenesis. They might also represent molecular biomarkers or targets for drug development in osteosarcoma.
Highlights
Osteosarcoma is the most common malignant bone tumor in childhood and adolescence
Realtime qPCR was used to evaluate the relative expression of 762 mature miRNA expression levels in 4 human tumors and 2 osteosarcoma cell lines (HOS and 143B) compared to a normal human osteoblast cell line (HOB)
With the objective of identifying miRNAs that are important in osteosarcoma tumorigenesis, we have performed miRNA expression profiling of two osteosarcoma cells lines as well as 4 formalin-fixed paraffin-embedded (FFPE) human tumor samples using Time PCR system in 384-well low-density arrays (TLDAs) arrays
Summary
Osteosarcoma is the most common malignant bone tumor in childhood and adolescence. Many tumors initially respond to chemotherapy, patients with metastatic disease and/or relapsed disease continue to have extremely poor survival outcomes [1]. Previous studies have demonstrated diverse genetic alterations in osteosarcoma cells, including structural abnormalities, gains and/or losses of chromosomes, as well as mutations in tumor suppressor genes [2]. Epigenetic modifications such as genomic DNA methylation and alterations of chromatin-associated proteins have been implicated in osteosarcoma carcinogenesis [3, 4]. The role of noncoding RNAs, especially microRNAs, in the initiation and progression of osteosarcoma is yet to be elucidated
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