Abstract
Blunt snout bream (Megalobrama amblycephala) is a widely favored herbivorous fish species and is a frequentlyused fish model for studying the metabolism physiology. This study aimed to provide a comprehensive illustration of the mechanisms of a high-starch diet (HSD) induced lipid metabolic disorder by identifying microRNAs (miRNAs) controlled pathways in glucose and lipid metabolism in fish using high-throughput sequencing technologies. Small RNA libraries derived from intestines, livers, and brains of HSD and normal-starch diet (NSD) treated M. amblycephala were sequenced and 79, 124 and 77 differentially expressed miRNAs (DEMs) in intestines, livers, and brains of HSD treated fish were identified, respectively. Bioinformatics analyses showed that these DEMs targeted hundreds of predicted genes were enriched into metabolic pathways and biosynthetic processes, including peroxisome proliferator-activated receptor (PPAR), glycolysis/gluconeogenesis, and insulin signaling pathway. These analyses confirmed that miRNAs play crucial roles in glucose and lipid metabolism related to high wheat starch treatment. These results provide information on further investigation of a DEM-related mechanism dysregulated by a high carbohydrate diet.
Highlights
Carbohydrates are an important source of energy and organic carbon for living organisms
Excessive glucose load in M. amblycephala caused a consequence of metabolic disorders and dysfunctions in biological processes and molecular pathways in glucose and lipid metabolisms, including the peroxisome proliferator-activated receptor (PPAR) pathway [11,12,13], the cyclic adenosine monophosphate(cAMP) response element-binding protein [14], and the insulin signaling pathway [12,13,15], which associate with metabolism regulation
Independent small RNA libraries were generated from M. amblycephala intestines, livers and brains fed with normal starch diet (34%, normal-starch diet (NSD)) and high starch diet (51%, high-starch diet (HSD)) groups using the Illumina Hiseq 2500 platform (Illumina, San Diego, CA, USA) to identify miRNAs involved in glucose metabolism
Summary
Carbohydrates are an important source of energy and organic carbon for living organisms. Excessive glucose load in M. amblycephala caused a consequence of metabolic disorders and dysfunctions in biological processes and molecular pathways in glucose and lipid metabolisms, including the peroxisome proliferator-activated receptor (PPAR) pathway [11,12,13], the cyclic adenosine monophosphate(cAMP) response element-binding protein [14], and the insulin signaling pathway [12,13,15], which associate with metabolism regulation This dysregulation could be regulated by abnormal expression of genes and microRNAs (miRNAs, single-stranded non-coding RNA molecules length at 18–25 nucleotides) in response to those stimuli
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