Abstract
Identification of Differentially Expressed Long Noncoding RNAs and mRNAs Involved with Dominant Follicle Selection in Goats using RNA-seq
Highlights
The experimental conditions of this study were approved by the Committee on the Ethics of Animal Experiments of the Southwest University (No [2007] 3) and the Animal Protection Law of China
Ovaries of three samples of Dazu black goats 1.1-1.2 years old were collected on October 12, 2015 at the domestic animal conservation field of the Southwest University (Chongqing, China; E106°25′29.19′′, N29°49′36.72′′, 763 m), and detail information about individuals in this suty as Supplementary Table I
The collected tissue was stored, and the follicles were stripped in 37°C saline immediately after removal
Summary
The experimental conditions of this study were approved by the Committee on the Ethics of Animal Experiments of the Southwest University (No [2007] 3) and the Animal Protection Law of China. Ovaries of three samples of Dazu black goats 1.1-1.2 years old were collected on October 12, 2015 at the domestic animal conservation field of the Southwest University (Chongqing, China; E106°25′29.19′′, N29°49′36.72′′, 763 m), and detail information about individuals in this suty as Supplementary Table I. The collected tissue was stored, and the follicles were stripped in 37°C saline immediately after removal. The follicles of 3 female individuals were separated into dominant (diameter > 5 mm) and nondominant follicles (3mm < diameter < 5mm) using the standard protocol (Medan et al, 2005; Supplementary Fig. S1)
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