Identification of Differentially Expressed Genes and Key Pathways Associated with PTPN11 Mutation in Acute Myeloid Leukemia

Abstract

PTPN11 is a prominent oncogene in many tumors, which linked with poor prognosis. But the gene expression profile associated with this gene mutation in AML is not yet fully elucidated. So, the current study was intended to explore the key genes and pathways associated with AML with PTPN11 mutation. Using the TCGA database, we investigated the gene expression profile for PTPN11 mutation and wild type. EdgeR, an R platform, was applied to categorize the differentially expressed genes. GO and KEGG enrichment analysis was accomplished using DAVID and GSEA. PPI network is investigated with STRING database and Cytoscape software. A statistical investigation was employed to examine the expression levels of genes and prognostic values. As a result, in total 3268 gene s were differentially expressed. GO, KEGG pathway and GSEA analysis of up and down-regulated genes represented that gene were enriched in apoptosis and metabolic signaling pathways. Furthermore, ten hub genes GAPDH, HSP90AA, MYC, UBB, HSPA4, EP300, CCDS, POLR2A, EFTUD2, RPL11. GAPDH was identified from the PPI network. A statistical investigation revealed that PTPN11 gene mutation associated with AML patients had a shorter median survival time with an increased risk of death compared with those without mutations.