Abstract
A rapid, specific, and novel gradient LC–MS method has been developed for the identification of degradation products (DPs) of cyclophosphamide (CY) under different stress conditions using liquid chromatography combined with quadrupole time-of-flight electrospray ionization tandem mass spectrometry (LC-QTOF–ESI-MS/MS). CY was subjected to hydrolytic (acidic, alkaline, and neutral) as per ICH guidelines Q1A (R2). The drug showed extensive degradation in hydrolytic stress conditions. In total, eight DPs were formed, and the chromatographic separation of drug and its DPs was achieved on a C-8 column (2.1 × 100 mm, 1.7 µm) using gradient elution method. The elemental composition of the degradation products was elucidated by using LC–ESI–MS/MS combined with accurate mass measurements.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Journal of Liquid Chromatography & Related Technologies
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.