Abstract
The high recurrence and improved survival rate of clear-cell renal cell carcinoma (ccRCC) demand for continuous effort to search for novel prognostic factors. Herein a comparative proteomics approach based on two-dimensional polyacrylamide gel electrophoresis (2-DE) and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was used to identify differentially expressed proteins between ccRCC cell line RLC-310 and normal renal cell line HK-2. Of the 31 proteins identified, Cyclophilin A (CypA) was a newly identified differentially expressed protein in ccRCC cell line. The overexpression of CypA in ccRCC tissues was confirmed using RT-PCR and Western blot analyses. Further immunohistochemistry revealed that overexpression of CypA was associated with poor differentiation and decreased survival (p
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
