Abstract
The extraction of endogenous 3′,5′-cyclic-AMP, 3′,5′-cyclie-GMP, 3′,5′-cyclic-CMP, 3′,5′-cyclic-UMP, 3′,5′-cyclic-IMP and 3′,5′-cyclic-deoxyTMP from meristematic and non-meristematic regions of Pisum roots is described. Extracts were subjected to a sequential purification procedure involving adsorption chromatography on alumina, ion-exchange chromatography, and preparative electrophoresis. Purified samples were compared with cyclic nucleotide standards by HPLC and UV absorbance spectrophotometry; further analysis was carried out by fast atom bombardment mass spectrometry and mass-analysed ion kinetic energy spectrometry. 3′,5′-Cyclic-AMP, -GMP, -CMP and -UMP were found to be present in Pisum meristematic tissue and 3′,5′-cyclic-AMP, -GMP, -CMP, -IMP and -DTMP are present in the non-meristematic regions. Data indicate that there is a significantly higher concentration of cyclic CMP in meristematic tissue relative to that in non-meristematic regions.
Published Version
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