Abstract
Coxiella burnetii is the causative agent of Q fever in humans and animals. This study aimed to determine the frequency of C. burnetii in milk samples of dairy animals (goats, sheep, and cattle) in some selected regions in Iran, where there is no information about prevalence of C. burnetii. In this study, 162 individual milk samples were collected from 43 farms in three provinces (Tehran, Hamadan, and Mazandaran). Real-time PCR was used for the detection of IS1111a element of C. burnetii. In total, 23 of 162 samples (14.2%, 95% confidence interval (CI): 9.65–20.2%) were positive for C. burnetii by real-time PCR. C. burnetii was detected in 10.17% (95% CI: 4.74–20.46) of goat milk samples. In sheep milk samples, 18.6% (95% CI: 9.74–32.62) were positive, and C. burnetii was detected in 15% (95% CI: 8.1–26.11) of cattle milk samples. Molecular evidence of the presence of C. burnetii was seen in milk samples of dairy animals in all the studied regions. These findings demonstrated that C. burnetii infection, especially in raw milk samples, deserves more attention from the health care system and veterinary organization in Iran.
Highlights
Coxiella burnetii is a fastidious and obligate intracellular bacterium which is the causative agent of Q fever in humans and animals
All milk samples were processed in the laboratory to remove of cream and precipitation cells based on previously published protocols [9]. e final precipitate was dissolved in 1 mL phosphate-buffered saline (PBS) solution and preserved at −20°C until DNA extraction
C. burnetii was detected in 10.17% of goat milk samples. e prevalence of C. burnetii in Mazandaran and Tehran provinces was 11.11% and 10%, respectively (Table 1)
Summary
Ashraf Mohabati Mobarez, Ehsan Mostafavi, Mohammad Khalili, and Saber Esmaeili 2,3. Is study aimed to determine the frequency of C. burnetii in milk samples of dairy animals (goats, sheep, and cattle) in some selected regions in Iran, where there is no information about prevalence of C. burnetii. Real-time PCR was used for the detection of IS1111a element of C. burnetii. 23 of 162 samples (14.2%, 95% confidence interval (CI): 9.65–20.2%) were positive for C. burnetii by real-time PCR. C. burnetii was detected in 10.17% (95% CI: 4.74–20.46) of goat milk samples. 18.6% (95% CI: 9.74–32.62) were positive, and C. burnetii was detected in 15% (95% CI: 8.1–26.11) of cattle milk samples. Molecular evidence of the presence of C. burnetii was seen in milk samples of dairy animals in all the studied regions. Ese findings demonstrated that C. burnetii infection, especially in raw milk samples, deserves more attention from the health care system and veterinary organization in Iran Molecular evidence of the presence of C. burnetii was seen in milk samples of dairy animals in all the studied regions. ese findings demonstrated that C. burnetii infection, especially in raw milk samples, deserves more attention from the health care system and veterinary organization in Iran
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