Abstract
Thinopyrum elongatum (Host) D.R. Dewey has served as an important gene source for wheat breeding improvement for many years. The exact characterization of its chromosomes is important for the detailed analysis of prebreeding materials produced with this species. The major aim of this study was to identify and characterize new molecular markers to be used for the rapid analysis of E genome chromatin in wheat background. Sixty of the 169 conserved orthologous set (COS) markers tested on diverse wheat-Th. elongatum disomic/ditelosomic addition lines were assigned to various Th. elongatum chromosomes and will be used for marker-assisted selection. The macrosyntenic relationship between the wheat and Th. elongatum genomes was investigated using EST sequences. Several rearrangements were revealed in homoeologous chromosome groups 2, 5, 6 and 7, while chromosomes 1 and 4 were conserved. Molecular cytogenetic and marker analysis showed the presence of rearranged chromosome involved in 6ES and 2EL arms in the 6E disomic addition line. The selected chromosome arm-specific COS markers will make it possible to identify gene introgressions in breeding programmes and will also be useful in the development of new chromosome-specific markers, evolutionary analysis and gene mapping.
Highlights
Wild relatives of bread wheat (Triticum aestivum L.) are important sources of agriculturally useful traits and alleles for wheat improvement
When the hybridization pattern of the 6E chromosome detected in the addition line was compared to those of the amphiploid line (Fig 2), it was found that the fluorescence in situ hybridization (FISH) pattern of the 6E long arm in the addition line was highly similar to the long arm of 2E chromosome present in the amphiploid line, in the 2E addition line or in the 2EL ditelosomic line (Fig 1; S3 Fig)
The present study showed that the wheat-Th. elongatum 6E disomic addition line contains a 6ES-2EL translocation
Summary
Wild relatives of bread wheat (Triticum aestivum L.) are important sources of agriculturally useful traits and alleles for wheat improvement. Wild gene variants can be transferred by interspecific or intergeneric hybridization in order to increase the allelic diversity of wheat [1]. Species from the Thinopyrum genus have long been used as genetic sources of salinity, drought and low temperature tolerance [2,3] and high grain micronutrient and protein content [4,5,6]. Several genes for resistance to leaf and stem rusts, wheat streak mosaic virus, barley yellow. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript
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