Identification of Conserved miRNAs in Solanum Lycopersicum Response to Long-term RPM-treatment

Abstract

 Abstract—T o identify the miRNAs associated with the simulated microgravity response in plants and to ascertain the regulation network mediated by miRNAs under simulated microgravity conditions, we constructed a miRNA library by direct cloning method and analyzed the library. Six conserved Solanum lycopersicum miRNAs were identified for the first time in Solanum lycopersicum under simulated microgravity condition. Gene ontology analysis showed that most of the predicted targeted genes were involved in organelle part, transcription factor, signal transduction and metabolic process, implying a complicated relationship among the external signal, internal transduction and final phenotype. Cis-elements located in the upstream sequences of each miRNA were identified and their roles in gene regulation were investigated. In the study, miRNAs were identified in S. lycopersicum for the first time under long-term simulated microgravity condition, which will help reveal the regulation mechanism mediated by miRNAs under simulated microgravity condition and adaptation to Earth’s gravity.