Abstract
Identification of conserved and novel mature miRNAs in selected crops as future targets for metabolic engineering
Highlights
A large number of miRNA have been reported in Glycine max (756), Oryza sativa (738), Zea mays (325), Sorghum bicolor (241), Brassica napus (92), Triticum aestivum (125), in miRBase (Griffiths-Jones et al, 2007)
A phylogenetic model was employed in Oryza sativa with the aim of identifying all identical miRNA sequence (Figure 3)
The same phylogenetic model was employed in Zea mays to identify identical miRNA sequence (Figure 4)
Summary
Energy has become the basic necessity in the social and economic development as well as in improving the standards of human being. Biomass from agricultural practices (bagasse, wheat/rice straw), wastewater cultivated microalgae (Shahid et al, 2020) and biomass from non-arable lands has shown promising potential as a renewable and lowcost feedstock to produce energy either biological or thermochemical processes (Mehmood et al, 2017, Ahmad et al, 2017, Ye et al, 2018). Among various targets of metabolic engineering, Micro-RNAs have come forward as targets of interest due to their diverse roles in plant physiology including biomass production (Joshi et al, 2017). MicroRNAs (miRNA) are small non-coding RNAs, comprising about 22 nucleotides (Zhang et al, 2006b) and perform diverse physiological roles in the development of plant ;(Nogueira et al, 2007, Chitwood et al, 2009, RubioSomoza et al, 2009), abiotic and biotic stress responses (Shukla et al, 2008, Ruiz-Ferrer and Voinnet, 2009), signal transduction, protein degradation (Guo et al, 2005, Zhang et al, 2006b), post-transcriptional gene expression, and cellular metabolism (Zhang et al, 2006b, Zhao et al, 2010). Study was aimed to propose the targets, those can be used in future for the metabolic engineering as well as in biomass production to meet the need of energy crises in future
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