Abstract
Purpose : As part of our ongoing research on bioactive compounds from Brazilian plants for the treatment of tropical diseases, we have investigated the species Miconia ibaguensis . Methods : The leaves of M. ibaguensis were dehydrated at 40°C, powdered and extracted by maceration with ethanol/water (96:4 v/v) at room temperature. The hydroalcoolic extract was particioned with n- hexane, ethyl acetate and n -butanol. We have employed GC-MS to investigate the n -hexane fraction. The method here reported is rapid and simple and does not require previous derivatization of the crude botanical extract. Also, the crude extract and the fractions were evaluated against trypomastigostes forms of Trypanosoma cruzi . Results : The GC-MS analysis of the n -hexane fraction showed that the major compounds are: palmitic acid ethyl ester, phytol, oleic acid, 4,8,12,16-tetramethylheptadecan-4-olide, 6,10,14-trimethyl-2-pentadecanona. The crude extract and n- hexane fraction showed an IC 50 of 101.8µg/mL and IC 50 >200.0µg/mL, respectively, against trypomastigostes forms of T. cruzi. Conclusion : In summary, we have reported here a simple and rapid method to identify compounds from non-polar bioactive botanical extracts and their fractions of Miconia species using GC-MS.
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