Abstract
As a warm climate species, soybean is highly sensitive to chilling temperatures. Exposure to chilling temperatures causes a significant reduction in the nitrogen fixation rate in soybean plants and subsequent yield loss. However, the molecular basis for the sensitivity of soybean to chilling is poorly understood. In this study, we identified cold-responsive miRNAs in nitrogen-fixing nodules of soybean. Upon chilling, the expression of gma-miR397a, gma-miR166u and gma-miR171p was greatly upregulated, whereas the expression of gma-miR169c, gma-miR159b, gma-miR319a/b and gma-miR5559 was significantly decreased. The target genes of these miRNAs were predicted and validated using 5' complementary DNA ends (5'-RACE) experiments, and qPCR analysis identified putative genes targeted by the cold-responsive miRNAs in response to chilling temperatures. Taken together, our results reveal that miRNAs may be involved in the protective mechanism against chilling injury in mature nodules of soybean.
Highlights
Cold stress, including chilling (
It has been shown that the Lb content of plant nodules is adversely affected by various abiotic stresses, including drought, cadmium, sodium and nitrite, and by senescence in many legumes, such as Lupinus albus, Pisum sativum, Medicago truncatula, Lotus japonicus and soybean [28,29,30,31,32,33]
The expression levels of gma-miR169c, gma-miR159b and gma-miR319a/b were reduced by approximately 50%. These results suggest that miRNAs and miRNA-mediated molecular pathways modulate nitrogen fixation in low temperature-treated nodules
Summary
Cold stress, including chilling (
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