Identification of coexisting pentose, hexose, and disaccharides with preliminary separation through hydrophilic interaction on silica HPTLC plate using aqueous sodium deoxycholate-acetonitrile mobile phase system

Abstract

Aqueous micellar bile salt, sodium deoxycholate (NaDC) solution as additive in acetonitrile (ACN) in the ratio (1:5 ν/ν) was identified as the most favorable mobile phase for on-plate identification and resolution of three series of common sugars (disaccharide, pentose and hexose) on commercially available high performance silica gel plates. Effect of mobile phase composition and the presence of heavy metal cations as impurities in the analyte sample have been investigated to optimize experimental conditions for the separation. The lowest possible amount of all sugar on high-performance thin-layer chromatography (HPTLC) plate has been determined at nanogram level. This method was successfully applied for identification and separation of sugars in pharmaceutical formulations [cough syrup (Honitus), multivitamin syrup (Becozinc)] and biological matrix (human blood).