Abstract
BackgroundCircular RNAs (circRNAs) are a novel class of noncoding RNAs. Increasing evidence indicates that circRNAs play an important role in the occurrence and development of tumors. However, the role of circRNA hsa_circ_0044556 in the progression of colorectal cancer (CRC) remains unclear.MethodsFirst, we searched for differentially expressed circRNAs using a circRNA microarray in paired CRC and adjacent normal tissues. The circRNA hsa_circ_0044556 was screened out from the existing CRC circRNA microarray in the Gene Expression Omnibus database and our microarray. The clinical significance of hsa_circ_0044556 expression level in CRC patients was then investigated. Finally, the functions of the targets of this circRNA were determined in CRC cell lines.ResultsHsa_circ_0044556 was highly expressed in CRC patients and was positively correlated with tumor stage and lymph node metastasis. In CRC cell lines, the proliferation, migration, and invasion of cancer cells were inhibited by knocking down hsa_circ_0044556 expression.ConclusionHsa_circ_0044556 promoted the progression of CRC. It is possible that hsa_circ_0044556 will become a novel biomarker or therapeutic target for CRC.
Highlights
Circular Ribonucleic acid (RNA) are a novel class of noncoding RNAs
The unsupervised hierarchical clustering shows that circRNAs had different expression in colorectal cancer (CRC) vs. adjacent normal tissues (Fig. 1b)
The differential expression of these circRNAs was further confirmed in the volcano plot
Summary
Circular RNAs (circRNAs) are a novel class of noncoding RNAs. Increasing evidence indicates that circRNAs play an important role in the occurrence and development of tumors. The role of circRNA hsa_ circ_0044556 in the progression of colorectal cancer (CRC) remains unclear. Circular RNAs (circRNAs) are a new class of noncoding RNAs (ncRNAs), which are single-stranded circular RNAs with no free 5′-end cap or 3′-end poly (A) tail. They are produced by alternative splicing of a specific pre-mRNA (pre-mRNA). CircRNAs are highly stable in cells with high expression, and sometimes their expression levels are even 10 times higher than those of their homologous messenger RNAs. It has been found that circRNAs can act as a miRNA sponge bound to RNA-binding protein to exert biological functions
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