Abstract
Colorectal cancer (CRC) is the third most common malignancy worldwide. Circular RNAs (circRNAs) have been implicated in cancer biology. The purpose of the current work is to investigate the precise parts of circRNA centrosome and spindle pole-associated protein 1 (circ-CSPP1) in the progression of CRC. Our data showed that circ-CSPP1 was significantly overexpressed in CRC tissues and cells. The knockdown of circ-CSPP1 attenuated cell proliferation, migration, invasion and promoted apoptosis in vitro and weakened tumor growth in vivo. circ-CSPP1 directly targeted miR-431, and circ-CSPP1 knockdown modulated CRC cell progression in vitro via upregulating miR-431. Moreover, LIM and SH3 protein 1 (LASP1) was a functional target of miR-431 in modulating CRC cell malignant progression. Furthermore, circ-CSPP1 in CRC cells functioned as a posttranscriptional regulator on LASP1 expression by targeting miR-431. Our present study identified the oncogenic role of circ-CSPP1 in CRC partially by the modulation of the miR-431/LASP1 axis, providing evidence for circ-CSPP1 as a promising biomarker for CRC management.
Highlights
Colorectal cancer (CRC) is the third most prevalent malignancy worldwide as of 2018, accounting for approximately one in ten cancer cases and deaths [1]
Our present study identified the oncogenic role of circ-CSPP1 in CRC partially by the modulation of the miR-431/LIM and SH3 protein 1 (LASP1) axis, providing evidence for circ-CSPP1 as a promising biomarker for CRC management
The precise actions of circRNAs in CRC development have remained unclear. circ-CSPP1 was recently identified as a circRNA whose overexpression in CRC contributed to the stemness of CRC cells [10]
Summary
Colorectal cancer (CRC) is the third most prevalent malignancy worldwide as of 2018, accounting for approximately one in ten cancer cases and deaths [1]. Biomarkers are considered as important tools in early detection and clinical therapy of patients with CRC [3]. There is an urgent need to identify novel and effective biomarkers for managing CRC. Circular RNAs (circRNAs), mostly formed by head-totail splicing of exons, are an important family of posttranscriptional regulators in eukaryotes [4]. CircRNAs are attracting considerable attention due to their microRNA (miRNA) regulation in almost all cancers, including CRC [5,6]. Yuan et al highlighted hsa_circ_ 0026344 as a novel prognostic biomarker for CRC patients by regulating miR-21 and miR-31 [7]. Li et al identified hsa_circ_0006990 as a negative regulator of miR-101, with the ability to accelerate CRC tumorigenesis [8]. Bian et al illuminated that hsa_circ_103809 controlled the carcinogenesis of CRC through targeting miR-532-3p [9]. No insight has been gained into the precise parts of circCSPP1 in CRC progression
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