Identification of Chromosome Segment Substitution Lines of Gossypium barbadense Introgressed in G. hirsutum and Quantitative Trait Locus Mapping for Fiber Quality and Yield Traits.

Huanchen Zhai,Huanchen Zhai,Huanchen Zhai,Junwen Li,Linglei Kong,Linglei Kong,Weiwu Song,Weiwu Song,Tingting Chen,Tingting Chen,Aiying Liu,Qun Ge,Youlu Yuan,Yunna Tan,Yunna Tan,Wankui Gong,Haihong Shang,Zhuying Deng,Zhuying Deng,Juwu Gong,Yuzhen Shi,David D Fang

doi:10.1371/journal.pone.0159101

Abstract

Chromosome segment substitution lines MBI9804, MBI9855, MBI9752, and MBI9134, which were obtained by advanced backcrossing and continuously inbreeding from an interspecific cross between CCRI36, a cultivar of upland cotton (Gossypium hirsutum) as the recurrent parent, and Hai1, a cultivar of sea island cotton (G. barbadense) as the donor parent, were used to construct a multiple parent population of (MBI9804×MBI9855)×(MBI9752×MBI9134). The segregating generations of double-crossed F1 and F2 and F2:3 were used to map the quantitative trait locus (QTL) for fiber quality and yield-related traits. The recovery rate of the recurrent parent CCRI36 in the four parental lines was from 94.3%–96.9%. Each of the parental lines harbored 12–20 introgressed segments from Hai1across 21 chromosomes. The number of introgressed segments ranged from 1 to 27 for the individuals in the three generations, mostly from 9 to 18, which represented a genetic length of between 126 cM and 246 cM. A total of 24 QTLs controlling fiber quality and 11 QTLs controlling yield traits were detected using the three segregating generations. These QTLs were distributed across 11 chromosomes and could collectively explain 1.78%–20.27% of the observed phenotypic variations. Sixteen QTLs were consistently detected in two or more generations, four of them were for fiber yield traits and 12 were for fiber quality traits. One introgressed segment could significantly reduce both lint percentage and fiber micronaire. This study provides useful information for gene cloning and marker-assisted breeding for excellent fiber quality.

Highlights

Cotton is one of the most important cash crops in the world and cotton fiber provides the main natural raw material for the textile industry
The absolute value of the skewness was
The recovery rates to the recurrent parent CCRI36 for fiber length (FL), fiber uniformity (FU), fiber micronaire (FM), and fiber strength (FS) increased from F1 to F2:3, whereas those of boll weight (BW) and lint percentage (LP) decreased

Summary

Introduction

Cotton is one of the most important cash crops in the world and cotton fiber provides the main natural raw material for the textile industry. Upland cotton (Gossypium hirsutum) has a high yield and wide adaptability, but a relatively low fiber quality. Sea island cotton (G. barbadense) has excellent fiber quality with low yield and limited adaptability. The simultaneous improvement of cotton fiber quality and yield is a tall task for breeders in conventional breeding [4]. With the continuous development and improvement of molecular marker technologies, researchers have conducted extensive studies to construct cotton genetic maps and identify quantitative trait locus (QTL). This would make it possible to simultaneously improve both of fiber quality and yield in a breeding program

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Results

Conclusion