Abstract
Changes in gene expression were examined in floral buds of a dormant woody perennial, blueberry ( Vaccinium, section Cyanococcus), in response to chilling unit accumulation. The concentration of 65, 60, and 14 kDa polypeptides increased in response to chilling under field conditions and with storage at 4–7°C. Levels of the polypeptides increased most dramatically during the first 300 h of chilling and decreased to the initial levels with the resumption of growth. The p Is, determined by isoelectric focusing of bud proteins, were 7.5–8.0 for the 65 and 60 kDa polypeptides and 8.5 for the 14 kDa polypeptide. Purification of the 65 and 60 kDa polypeptides following by amino acid sequence analysis of selected peptide fragments revealed similarities in amino acid composition between these polypeptides and dehydrins. Antiserum, raised against the lysine rich consensus sequence EKKGIMDKIKEKLPG of dehydrins, cross-reacted to all three of the chilling-responsive polypeptides of blueberry. Thus, the accumulation of dehydrin-like proteins in response to chilling was demonstrated in a woody perennial.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
