Identification of chicken GnRH II in brains of metatherian and early-evolved eutherian species of mammals

Abstract

Two molecular forms of GnRH (chicken GnRH II and a second variant) are present in the brains of species from all the major vertebrate groups. In mammals, two forms are present in metatherian species and early-evolved eutherian species, but chicken GnRH II has not been identified in more advanced eutherian species. We investigated the nature of GnRH molecular forms in several early-evolved mammalian species, using high performance liquid chromatography and radioimmunoassay with specific GnRH antisera. These chromatographic and immunological data indicate that in the brains of a metatherian species (possum, Trichosurus vulpecula) and in two early-evolved eutherian species (order Insectivora: musk shrew, Suncus murinus and mole, Chrysochloris asiatica), both mammalian and chicken II GnRHs are present, while in another relatively early-evolved eutherian species (order Chiroptera: bat, Miniopterus schreibersii) only mammalian GnRH is present. In the adult possum and mole brains the proportion of chicken GnRH II was lower than that of mammalian GnRH, while in the musk shrew brain chicken GnRH II predominated. A peptide likely to be mammalian proGnRH was detected in the brains of the three eutherian species (musk shrew, mole, and bat). These findings suggest that metatherian and primitive eutherian species of mammals continue to express chicken GnRH II as in the vast majority of nonmammalian vertebrates, while the peptide is apparently not expressed in modern placental mammalian species. The functional significance of chicken GnRH II is not yet clear, but there are indications that it has a neurotransmitter or neuromodulator role in addition to that of regulating pituitary hormone release in certain vertebrate species.