Identification of chemical structure of anthocyanin and other active substances of red color melinjo peels by FTIR and LC-MC analysis

Abstract

Research on red color melinjo peels containing anthocyanin active substance has been studied since 2011, starting from anthocyanin extraction stage (maceration) by using both polar and non-polar solvents, a variation of citric acid addition and stability of anthocyanin extract storage and application of anthocyanin for food and beverage. However, research on the identification of anthocyanin chemical structures and other active substances of red color melinjo peels is also necessary. The purpose of this study was to identify the structure of anthocyanin and other active substances from red color melinjo peels with FTIR and LC-MS analysis. The results obtained: from FTIR analysis shown wavelength, among others: 3425 cm-1, 1725 cm-1, 1632 cm-1 and 1219 cm-1, it shows that inside the red color melinjo peels contained OH function group, C=O, C=C, and CO. From the LC-MS analysis shown at a minute of 1.62 observed BM = 302.236, which is the molecular weight of the Quercetin compound. The mass spectrum with the base peak height of m/z = 165, at min 3.83 has 100% abundance, whereas the 50% abundance at peak M+m/z = 242 has BM = 271 with the anthocyanin molecule of red color melinjo peels C15O5H11. The anthocyanin type of red color melinjo peels is a cyanine compound.