IDENTIFICATION OF CHEMICAL COMPOUNDS AND ANTIBACTERIAL ACTIVITY TEST OF Kirinyuh LEAF EXTRACT (Chromolaena odorata L.) FROM Ie Seum GEOTHERMAL AREA, REGENCY OF ACEH BESAR, INDONESIA

Abstract

This research aims to identify and compare the chemical components of Chromolaena odorata leaf extract grown in the geothermal and outer areas of the geothermal source site. The antibacterial activity of the plant extracts was also identified. The plant materials were collected from the Ie Seum Geothermal area and Blangkrueng region, approximately 31 km from the geothermal area. All samples were extracted using three different solvents: ethanol, nhexane, and ethyl acetate. A phytochemical test was performed to identify the secondary metabolites, volatile components were identified using GC-MS, and the inhibition zone method was used to assess the antibacterial activity of the extracts against S. aureus. The ethanol extract and ethyl acetate from both areas contained phenolic, flavonoid, and steroid, while the n-hexane extracts from both areas’ steroid groups were identified. In addition, GCMS data revealed that the plant material sample from the geothermal contained more chemical constituents compared to the sample from the geothermal outer area. The extract from geothermal also shows better antibacterial activity against S. aureus, and statistical analysis indicates a significant difference in antibacterial activity among solvents and between the origin of the samples.