Abstract
Methods based upon the polymerase chain reaction (PCR) have been developed for distinguishing cereal species and cultivars. DNA isolated from the leaves of germinating seedlings or grain gave similar results. Primers designed to amplify the spacers between 5S ribosomal RNA genes were able to identify samples of maize, barley, sorghum, rye, rice, oat and wheat without any significant variation within these species. Arbitrary 10-mers allowed distinction between cultivars of the same species, although some variation was found within cultivars, and some primers gave products characteristic of the cereal species. These methods have potential for application in the analysis of the species and cultivars composition of cereal grain lots and cereal products.
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