Abstract
Background: Recurrent locally advanced or metastatic head and neck squamous cell carcinoma (HNSCC) is associated with dismal prognosis because of its highly invasive behavior and resistance to conventional intensive chemotherapy. The identification of effective markers for early diagnosis and prognosis is important for reducing mortality and ensuring that therapy for HNSCC is effective. Chaperonin-containing TCP-1 3 (CCT3) folds cancer-related proteins to control carcinogenesis. The prognostic value and growth association of CCT3 and HNSCC remain unknown.Methods: The GEO, Oncomine and UALCAN databases were used to examine CCT3 expression in HNSCC. A few clinical HNSCC samples with normal tissues were used to detect CCT3 expression by using immunohistochemistry method. The TCGA-HNSC dataset was used to evaluate the association between expression of CCT3 and prognosis. The molecular mechanism was investigated with gene set enrichment analysis (GSEA). CCK-8 and wound healing assays were used to detect cell growth and invasion of HNSCC, respectively.Results: CCT3 expression was significantly up-regulated in HNSCC in both mRNA and protein levels. In addition, up-regulated CCT3 expression was associated with various clinicopathological parameters. High expression of CCT3 was significantly correlated with inferior survival of HNSCC patients. Knockdown of CCT3 significantly inhibited cell growth and invasion of HNSCC cell lines. GSEA analysis indicated that CCT3 was closely correlated with tumor-related signaling pathways and HNSCC cell survival.Conclusion: Our findings suggest that CCT3 is a biomarker of poor prognosis and related to the process of HNSCC.
Highlights
Head and neck cancer (HNC) is a common malignant worldwide and causes more than 600,000 new cases every year [1]
Because Chaperonin-containing TCP-1 3 (CCT3) was significantly up-regulated in Head and neck squamous cell carcinoma (HNSCC) in seven datasets, the association between CCT3 and HNSCC was subjected into subsequent exploration
These results suggested that CCT3 was highly expressed in HNSCC than in associated normal tissues
Summary
Head and neck cancer (HNC) is a common malignant worldwide and causes more than 600,000 new cases every year [1]. Rapid advances in methods of diagnosis and treatment, the prognosis of patients with HNSCC remains not optimistic [3]. Recurrent locally advanced or metastatic head and neck squamous cell carcinoma (HNSCC) is associated with dismal prognosis because of its highly invasive behavior and resistance to conventional intensive chemotherapy. Chaperonin-containing TCP-1 3 (CCT3) folds cancer-related proteins to control carcinogenesis. The prognostic value and growth association of CCT3 and HNSCC remain unknown. Results: CCT3 expression was significantly up-regulated in HNSCC in both mRNA and protein levels. High expression of CCT3 was significantly correlated with inferior survival of HNSCC patients. GSEA analysis indicated that CCT3 was closely correlated with tumor-related signaling pathways and HNSCC cell survival. Conclusion: Our findings suggest that CCT3 is a biomarker of poor prognosis and related to the process of HNSCC
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