Identification of Candidate Gene <i>SalT</i> and Designing Markers Involving in Salt Tolerance of Vietnamese Rice Landraces

Abstract

Rice (Oryza sativaL.) is a principle crop with great economic importance in Vietnam, providing daily food for over 90 million people in this country. However, a large rice growing areas and rice production are being seriously affected by the threats of devastation from climate change. The need to develop salinity tolerance rice varieties to cope with adverse climate change is very imperative. In this study, based on the genome sequence databases of 36 Vietnamese rice landraces, we have identified 9 Vietnamese rice landraces carrying nineSalTcandidate gene with the sequence similarity toO. sativa SalT(the published GenBank: Z25811.1) which have shown salinity tolerance are: Te Nuong, Khau mac buoc, Chan thom, Khau giang, Tan ngan, Nang thom cho dao, OM5629, Hom rau and Thom Lai). Amongst them, 4 rice landraces including Nang thom cho dao, OM5629, Hom rau and Thom lai have shown 2 fragments of deletion with 6 and 7 nucleotides which were most identical to the referenceSalTgene. Two primers has been successfully designed to identify theSalTcandidate gene in Vietnamese rice landraces. This study provides useful information of salinity tolerance of some Vietnamese rice landraces for breeding programs.