Abstract
Oral candidiasis is a condition commonly seen in patients with systemic diseases such as diabetes, a common and globally growing health problem associated with many risk factors. The objective of the study was to identify Candida albicans in cultures and compare various cytologic stains used for diagnosis of oral candidiasis. Ninety type 2 diabetic individuals were chosen for the study group. The control group contained 30 non-diabetic individuals. Random blood sugar levels were analyzed for all diabetic and non-diabetic patients. Gram stain, periodic acid-Schiff technique (PAS) and calcofluor white were used for staining buccal mucosa smears collected from all individuals. An oral rinse was collected from all subjects and inoculated on Sabouraud dextrose agar (SBA) to confirm Candida positivity. Results for Candida positivity from one patient show variability between the three stains. Cultures showed higher values of these variables i.e. specificity, sensitivity and positive predictive values for Candida identification as compared to the staining results. This study showed detection of C. albicans in the oral cavity was better using SBA culture media than stained exfoliative smears.
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