Abstract
Candida species are one of the most important causes of human infections, especially in hospitals and among immunocompromised patients. The correct and rapid etiological identification of yeast infections is important to provide adequate therapy, reduce mortality, and control outbreaks. In this study, Candida species were identified in patients with suspected fungal infection, and phenotypic and genotypic identification methods were compared. A total of 167 axenic fungal cultures and 46 clinical samples were analyzed by HardyCHROM®, MicroScan®(Omron Microscan Systems Inc, Renton, WA, USA), and PCR-RFLP (Restriction Fragment Length Polymorphisms). The species of the C. albicans complex were the most frequent, followed by C. tropicalis and C. glabrata. Less common but clinically relevant species of Candida were also isolated. The comparison between the three methods was concordant, especially for the most common Candida species. Fungal DNA amplification was successful in all clinical samples.
Highlights
Yeast infections vary in severity and can range from superficial infections [1] to sepsis with deadly potential [2]
Frequency of Candida Species According to HardyCHROM®
Phenotypic identification of yeasts was performed by chromogenic reaction in HardyCHROM®
Summary
Yeast infections vary in severity and can range from superficial infections [1] to sepsis with deadly potential [2]. One of the most relevant yeast genera due to its high morbidity and mortality is Candida. The Candida genus includes at least 15 species associated with human pathologies [3]. Most species of the genus Candida are considered normal inhabitants of the skin and mucous membranes. Under specific circumstances, these microorganisms have the potential to express virulence factors that make them pathogenic or opportunistic, in settings in which the patient suffers a debilitating clinical condition [4,5], or due to the use of invasive devices [6], or under immunosuppression [7]. The most severe candidiasis have a nosocomial origin and the source of infection can be both endogenous or exogenous [8]
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