Identification of BRCA1/2 p.Ser1613Gly, p.Pro871Leu, p.Lys1183Arg, p.Glu1038Gly, p.Ser1140Gly, p.Ala2466Val, p.His2440Arg variants in women under 45 years old with breast nodules suspected of having breast cancer in Burkina Faso.

Tani Sagna,Dorcas Obiri-Yeboah,Charlemagne Ouedraogo,Daniela Fusco,Virginio Pietra,Cyrille Bisseye,Marie Nabonswindé Lamoussa Ouedraogo,Daniela Turchetti,Zoenabo Douamba,Olga Melanie Lompo,Marco Seri,Jacques Gilbert Kafando,Elena Bonora,Florencia Djigma,Jacques Simpore,Abdou Azaque Zoure,Nayi Zongo

doi:10.1515/bmc-2019-0015

Abstract

Breast cancer is the top cause of cancer mortality among women in the world and the second in Africa. The aims of this study were to: i) identify women with breast nodules suspected of having breast cancer ii) sequence the BRCA1 and BRCA2 genes and iii) screen mutations. From 2015 to 2016, 112 women aged from 35 to 44 years, who had come for consultation in the gynecology/obstetrics and the oncology department of the University Hospital Yalgado Ouedraogo, voluntarily agreed to participate to this study. Whole blood was collected from those with mammary nodules. The genomic DNA was extracted using Qiagen kit. FAST KAPA was used for genomic DNA amplification and the purified PCR products were analyzed by direct sequencing using Big Dye v1.1 and ABI 3730 automated sequencer. Nucleotides substitutions were determined. We identified BRCA1 SNPs rs1799966, rs799917, rs16942, rs16941, rs2227945, and BRCA2 SNPs rs169547, rs4986860. These identified variants are found mostly in cases of benign tumors of breast or ovarian cancer with familial history of breast cancer. This study in Burkina-Faso, is the basis for improved and more specific genetic testing, and suggests that additional genes contributing to an increased risk of breast cancer should be analyzed.

Highlights

Breast cancer is the most common cancer in women, especially in those over 50 years old for whom it is the leading cause of death worldwide
Based on the eligibility criteria, 9 women were screened for genetic breast cancer
BRCA1 and BRCA2 genes have been screened in young women with nodules, suspected of being breast cancer, and some variants have been found

Summary

Introduction

Breast cancer is the most common cancer in women, especially in those over 50 years old for whom it is the leading cause of death worldwide. Breast cancer is the second most frequent cancer both in the totality of African countries and in the. Breast cancer is a malignant tumor of the mammary gland. This cancer is most common in women, with 89 cases per 100,000. In Burkina Faso (one of the west African countries) breast cancer is being responsible for 17.7% of women’s deaths [7]. A recent study provide preliminary information about breast cancer risk decreasing with multiparity among women in Burkina Faso [8, 9]. Genetic and non-genetic factors are involved in the etiology of breast cancer

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