Abstract
In a previous communication ( Waisman, D.M., Smallwood, J.I., Lafreniere, D. and Rasmussen, H. (1983) Biochem. Biophys. Res. Commun. 116 , 435–441 ) we reported that chromatography of bovine brain 100,000 × g supernatant on diethylaminoethyl (DEAE) cellulose and analysis of resultant fractions by chelex competitive calcium binding assay, resolved three peaks of calcium binding activity. Gel permeation chromatographic analysis of each peak resolved apparent M r 40,000 (Peak I), M r 75,000, M r 230,000 and M r 420,000 (Peak II), and M r 38,000 (Peak III). In the present communication the calcium binding proteins responsible for the calcium binding activity peaks resolved by gel permeation chromatography, have been purified and identified as caligulin, (M r 40,000), calcineurin, (M r 230,000) and calmodulin, (M r 38,000). In addition, a novel calcium binding protein(M r 48,000 by SDS PAGE) has been identified from the M r 75,000 calcium binding activity peak.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
