Abstract
BackgroundRice blast is an economically important and mutable disease of rice. Using host resistance gene to breed resistant varieties has been proven to be the most effective and economical method to control rice blast and new resistance genes or quantitative trait loci (QTLs) are then needed.ResultsIn this study, we constructed two advanced backcross population to mapping blast resistance QTLs. CR071 and QingGuAi3 were as the donor parent to establish two BC3F1 and derived BC3F2 backcross population in the Jin23B background. By challenging the two populations with natural infection in 2011 and 2012, 16 and 13 blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi3 population, respectively. Among Jin23B/CR071 population, 3 major and 13 minor QTLs have explained the phenotypic variation from 3.50% to 34.08% in 2 years. And, among Jin23B/QingGuAi3 population, 2 major and 11 minor QTLs have explained the phenotypic variation from 2.42% to 28.95% in 2 years.ConclusionsSixteen and thirteen blast resistance QTLs were identified in Jin23B/CR071 and Jin23B/QingGuAi3 population, respectively. QTL effect analyses suggested that major and minor QTLs interaction is the genetic basis for durable blast resistance in rice variety CR071 and QingGuAi3.
Highlights
Rice (Oryza sativa) is a staple food crop for more than 50% of the world’s population
simple sequence repeat (SSR) Assay of Two Backcross Population Two backcross populations derived from the cross between Jin23B and CR071 and between Jin23B and QingGuAi3 contained 239 and 237 plants, respectively
Two Quantitative trait loci (QTLs) qBR2–3 and qBR8 were detected in both year for leaf blast and neck blast resistance; five QTLs qBR2–1, Fig. 3 Distribution of QTLs for blast resistance in the Jin23B/CR071 population on the genetic linkage map. 11RT and 12RT, leaf blast resistance at tillering stage in 2011 and 2012. 11RH and 12RH, leaf blast resistance at heading stage in 2011 and 2012. 11RN and 12RN, neck blast resistance at maturation stage in 2011 and 2012
Summary
Rice (Oryza sativa) is a staple food crop for more than 50% of the world’s population. 37 genes have been cloned (Wang et al 2017; Wang et al 2019; Zhao et al 2018), and most of them belong to the nucleotide-binding site (NBS) leucine-rich repeat (LRR) gene family. Many of these R genes are clustered in the rice genome, especially on chromosomes 6, 11, and 12. Using host resistance gene to breed resistant varieties has been proven to be the most effective and economical method to control rice blast and new resistance genes or quantitative trait loci (QTLs) are needed
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