Abstract
The Pt(II) complex, PtCl2(DIP) (DIP = chelating dinitrogen ligand: 4,7-diphenyl-1,10-phenanthroline), was synthesized and characterized by elemental analysis (CHN) and 1H NMR and UV-vis techniques. The binding of this complex to calf thymus DNA was investigated using various physicochemical methods such as spectrophotometric, circular dichroism, spectrofluorometric, melting temperature, and viscosimetric techniques. Upon addition of the complex, important changes were observed in the characteristic UV-Vis bands (hyperchromism) of calf thymus DNA (CT-DNA): increase in melting temperature, sharp increase in specific viscosity of DNA, and induced CD spectral changes. Also the fluorescence spectral characteristics and interaction of Pt complex with DNA have been studied. Pt bound to DNA showed a marked decrease in the fluorescence intensity. The results show that both the complex and the NR molecules can intercalate competitively into the DNA double-helix structure. The experimental results show that the mode of binding of the this complex to DNA is classical intercalation.
Highlights
The interaction of transition metal complexes with DNA has long been the subject of intense investigation in relation to the development of new reagents for biotechnology and medicine [1,2,3]
The interaction of Pt (II) complex with calf thymus DNA (CT-DNA) has been monitored in aqueous solution
Hypochromism results from the contraction of DNA in the helix axis, as well as from the change in conformation on DNA; in contrast, hyperchromism derives from damage to the DNA double-helix structure [27, 28]
Summary
The interaction of transition metal complexes with DNA has long been the subject of intense investigation in relation to the development of new reagents for biotechnology and medicine [1,2,3]. In these complexes, metal or ligands can be varied in an controlled way to facilitate the individual applications. The observation that neutral platinum coordination compounds inhibit division and cell growth has generated much interest in the potential value of inorganic drugs in the field of cancer chemotherapy [5,6,7,8,9,10]. Because of its severe side effects a need for new platinum complexes has arisen, and several new derivatives have been synthesized and tested against various tumor model systems, with the hope of discovering new drugs with improved properties [9]
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