Abstract
Species identification by DNA barcodes has become in recent years a very useful tool in molecular biology. But the degradation level shown in some samples, makes regular barcodes impractical for cases in which DNA is fragmented as it happens commonly in forensic casework. Therefore, the use of mini barcodes with sizes below 200bp is recommended to achieve optimal amplifications. The present study is focused on the identification of five Iberian Peninsula big game species, Red deer (Cervus elaphus), roe deer (Capreolus capreolus), wild boar (Sus scrofa), brown bear (Ursus arctos) and wolf (Canis lupus), using a pair of universal primers that amplify a 148bp Cytochrome B mini barcode. The analysis of amplicons was made using High-Resolution Melting and the results have shown a correct identification of the five big game species analyzed even in degraded samples.
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