Identification of beta-lactamase genes in Nitrofurantoin-resistant gram-negative bacteria isolated from pregnant women using molecular techniques

Abstract

The predominant infection among pregnant women is urinary tract infection (UTIs), which may lead to various complications. So, this investigation was to detect gram-negative UTIs isolates and beta-lactam resistance genes. The study included 100 (28.57%) inpatient pregnant women and 250 (71.42%) outpatient pregnant women treated at Al-Zahraa teaching hospital for children’s health and maternity, Al-Najaf City, Iraq. Finding showed that The majority of the age group was 31-40 years old which involved 80 (22.85%) growth and 45 (12.85%) no growth. Results also showed the high rate of bacteria among pregnant women in third-trimester was 127 (36.28%). According Kirby-Bauer methods, only 33/150 (22%) of gram-negative isolates were nitrofurantoin-resistant. Results of PCR amplification showed that all 33 out of 33 (100%) gram negative isolates had the ctx-m gene. Additionally, a significant prevalence of AmpC gene was discovered in 32 out of 33 isolates (96.96%). The veb gene, on the other hand, was only detected in P. aeruginosa isolates, with a prevalence of 1 out of 33 (3.03%). The PCR findings revealed the presence of oxa-like 5 genes in 12.12% (4/33) of the isolates. Although oxa-69 was not identified in this study.