Identification of Barley (Hordeum vulgare L. subsp. vulgare) Root Exudates Allelochemicals, Their Autoallelopathic Activity and Against Bromus diandrus Roth. Germination

Imen Bouhaouel,Laurent Franzil,Gaëtan Richard,Hajer Slim Amara,Marc Ongena,Patrick Du Jardin,Aurélie Gfeller,Marie-Laure Fauconnier

doi:10.3390/agronomy9070345

Imen Bouhaouel, Laurent Franzil + Show 6 more

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https://doi.org/10.3390/agronomy9070345

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Abstract

Crops with weed suppressive root exudates or the direct use of bioherbicidal allelochemicals is a new approach in integrated weed management systems. In this context, the allelopathic activity and chemical composition of root exudates from six genotypes (modern varieties and landraces) of barley were characterized. The phenolic acids appeared to be particularly implicated in the inhibitory action of barley root exudates against Bromus diandrus. The amount of these compounds was higher in sandy substrate than in sandy-clay-loam substrate. Ten phenolic acids and one phenylpropanoid derivative were present, in addition to saponarin, a newly identified flavonoid in barley root exudates. Seven compounds explaining variability in the inhibitory activity of barley roots (stepwise analysis) and one compound detected only in highly allelopathic genotypes were toxic against receiver plants. Most compounds had a greater inhibitory effect on the growth of great brome than the barley genotypes. The synergistic and/or additive effect of the eight compounds appeared to be the source of the toxicity. Benzoic acid, the mixture of compounds, saponarin and salicylic acid were the most efficient compounds against the great brome and the less aggressive against barley. Overall, the results revealed the allelopathic potential of the water-soluble compounds exuded by the roots of living barley plants. These compounds included saponarin, a flavonoid not yet recognized as a barley root allelochemical.

Highlights

The above- and below-ground organs of living plants emit a wide range of compounds into their environment [1,2]
Our objectives were to (i) assess the importance of phenolic acids, seen as a major class of allelochemicals [20], in the allelopathic potential of Tunisian barley root exudates in two types of soil substrates and (ii) profile the allelochemical compounds of barley genotypes exuded in sandy soil substrate, identifying potential water-soluble compounds with bioherbicide potential
The inhibitory action of barley root exudates seems to depend partly on total phenolic acids, as our experiments showed, but some individual compounds, including hordenine [21,23], a well-known barley allelochemical of roots, could be involved

Summary

Introduction

The above- and below-ground organs of living plants emit a wide range of compounds into their environment [1,2]. Due to the increasing number of herbicide-resistant weeds and the environmental and human health concerns about the use of synthetic herbicides, ‘allelopathy’ has been proposed as a new approach to the biological control of weeds, together with suitable crop management techniques. This phenomenon might be exploited by the cultivation of allelopathic species or the direct use of bioherbicidal allelochemicals. The identification of compounds with bioherbicidal proprieties, has not yet been described and deserves more attention

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