Abstract
Fifty bacterial isolates isolated from dairy product, skin and blood from cancer and kidney failure dialysis patients were identified to twenty two species and the following genera:- Brevibacterium, Corynebacterium, Arthrobacter, Actinomyces, Exiguobacterium, Kocuria, Micrococcus, Rothia, Rhodococcus using a set of phenetic characteristics. Twelve isolates of the different species from the genera Brevibacterium, Arthrobacter, Corynebacterium, Kocuria, Rhodococcus, Rothia were selected and probed for lin gene by polymerase chain reaction. One species Kocuria rhizophila which inhibited most of the tested organisms did not have lin gene in the chromosome, while, the species Corynebacterium glucuronolyticum, Arthrobacter comminsii, Arthrobacter oxydans have the lin gene. Our results found there wide distribution of the structural gene encoding this linocin M18 within coryneform bacteria and also in the genus Kocuria.
Highlights
Brevibacterium spp. are gram-positive irregular, slender, rod-shaped bacteria that display a marked rod- to-coccus cycle, non-acid-fast
Conventional identification using phenotypic descriptions identified the isolates to nine genera: Brevibacterium, Corynebacterium, Arthrobacter, Actinomyces, Exiguobacterium, Kocuria, Micrococcus, Rothia, Rhodococcus & 22 species, which showed different ability to produce bacteriocin against some gram positive bacteria and yeast as summarized in Table (1)
One isolate belonged to Kocuria rhizophila which inhibited most test organisms as in the Fig. 1 did not have lin gene in the chromosome as in the Figure (2)
Summary
Brevibacterium spp. are gram-positive irregular, slender, rod-shaped bacteria that display a marked rod- to-coccus cycle, non-acid-fast. The peptidoglycan contains meso-DAP as the principal diamino acid [1]. They are obligately aerobic and oxidative in their metabolism of carbohydrates. They are non-motile and salt-tolerant > 6.5% NaCl. They are non-motile and salt-tolerant > 6.5% NaCl They produce catalase and proteinases and characteristically produce methanethiol CH3SH from L-methionine [2]. The habitat of the Brevibacterium is primarily milk products, in which the bacteria contribute to the aroma and the color toes and other intertriginous areas and are believed to contribute to body odor, but are rare causes of human infections as opportunistic agents [2]
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