Identification of Bacteria Sample Plaque from Periodontal Disease Among Diabetic Patients

Abstract

Molecular identification of bacteria are very important to develop database gene bank in university. Databases of genes are very important to annotate a collection of nucleotide sequence and protein translation of oral bacteria. This research was conducted to identify bacteria that present in the plaque sample among diabetic patients collected from the Department of Periodontology and Community Oral health Universiti Sains Islam Malaysia (USIM). A total of bacteria was successfully isolated on Blood Agar from periodontal patients’ plaques. Colony Forming Unit (CFU/mL) was calculated to estimate the number of viable bacteria in a sample after performed serial dilution. Gram stained was done for all isolated samples to differentiate between Gram positive and negative organisms. About 2.07 x 10 7 CFU/mL of bacteria was successfully collected from periodontal plaques on diabetes patient. From the numbers, only 17 were selected to identify the species. Seven from the samples were Gram-positive bacteria, meanwhile ten samples were identified as Gram-negative bacteria. PCR products were isolated from the samples by using polymerase chain reaction analysis. Purification had been done to all PCR products before sent it for sequencing. Streptococcus mutans, Streptococcus gordonii, Streptococcus oralis, Streptococcus sanguinis, Streptococcus agalactiae, Streptococcus lutetiensis, Streptococcus downei,, Aggregatibacter actinomycetemcomitans, Klebsiella pneumoniae, Enterobacter clocae, Salmonella enterica subs enterica, Enterobacter ludwigii, Enterobacter mori, Enterobacter roggen kampii, Enterobacter cloacae, Enterobacter tabaci and Klebsiella pneumoniae were identified from all samples via DNA sequencing.