Identification of Ava I Polymorphisms in the Third Intron of GH Gene and Their Associations with Abdominal Fat in Chickens

Abstract

Growth hormone (GH) plays a diverse role in animals together with other hormones of somatotropic axis. In the current research, chicken GH (cGH) as a candidate gene affecting carcass traits was investigated in the chickens from 2 local chicken breeds [Mountainous Black-Bone (Wugu) and Caoke chicken] in the Sichuan province, 1 pure line of a quality chicken (Sanhuang chicken) from the Guangdong province, and commercial crossbreds. The RFLP method was used to identify polymorphisms of the cGH gene. Three restriction enzyme polymorphic sites were detected in the cGH gene. Sequence alignment from GenBank revealed 2 mutations in the third intron of the cGH gene, which were identified by the Ava I enzyme. Two novel Ava I polymorphic sites were genotypied in 240 chickens from the above-mentioned chicken populations. One EcoR V polymorphic site, the previously reported polymorphism, was also detected in these populations. Significant differences in allelic and genotypic frequencies among all the chicken populations were observed. In Ava I polymorphic sites, allele A2 and B1 had higher frequencies than allele A1 and B2, respectively. In EcoR V polymorphic sites, the frequency of allele N2 was higher than that of allele N1. Associations of polymorphisms of the cGH gene with carcass traits were analyzed by using a GLM procedure. Significant associations were found between Ava I genotypes or combined genotypes and abdominal fat weight and abdominal fat percentage (P ≤ 0.05). The allele A2 and B1 had a beneficial effect on increasing the live BW, breast muscle weight, and breast muscle percentage while decreasing the abdominal fat weight, abdominal fat percentage, and s.c. fat thickness. No significant associations were observed between EcoR V genotypes and carcass traits. In conclusion, the cGH gene may be a potential marker affecting the abdominal fat trait of chickens.