Abstract
Background Atrial fibrillation (AF) is the most common arrhythmia in the world. Long noncoding RNA (lncRNA) has been found to play an important role in cardiovascular diseases including heart failure, myocardial infarction, and atherosclerosis. However, the role of lncRNA in AF has rarely been studied. The purpose of this study is to identify the expression profile of lncRNA in AF patients, explore the function of lncRNA in AF, and provide a potential scientific basis for the treatment of AF in the future. Methods The lncRNA and mRNA expression profiles were obtained from the atrial appendage samples of GSE31821, GSE411774, GSE79768, and GSE115574 in the Gene Expression Omnibus (GEO) database. Functional analysis was performed via Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and Gene Set Variation Analysis (GSVA). The “CIBERSORT” R kit was used to analyze 22 immune cell infiltrates in AF and sinus rhythm (SR) patients. The “CORRPLOT” R package was used to analyze the immune correlation between lncRNA and immune cells. Results A total of 6 differentially expressed lncRNAs and 45 differentially expressed mRNAs were identified in the AF and SR groups. GO, KEGG, and GSVA results showed that abnormally expressed lncRNAs were involved in signaling pathways related to the atrium, including the Toll-like receptor signaling pathway and calcium signaling pathway. Immune cell infiltration analysis revealed that native B cells, follicular helper T cells, and resting dendritic cells may be involved in the AF process. In addition, LINC00844 was negatively correlated with resting dendritic cells. Conclusion The expression profile of lncRNA in AF patients was different from that in normal controls. The physiological functions of these differentially expressed lncRNAs may be related to the pathogenesis of AF, which provide a scientific basis for the prognosis and treatment of patients with AF.
Highlights
Atrial fibrillation (AF) often coexists with arterial hypertension [1]
A total of 6 Long noncoding RNA (lncRNA) were differentially expressed between the sinus rhythm (SR) group and the AF group, compared with SR groups: 4 lncRNAs were upregulated and 2 were downregulated in Download microarray datasets from Gene Expression Omnibus (GEO) database
In order to further explore the composition of immune cells in AF and SR groups, we investigated the immune cell infiltration landscape by using the CIBERSORT algorithm
Summary
Atrial fibrillation (AF) often coexists with arterial hypertension [1]. Generally, AF is positively correlated with age [2]. Long noncoding RNA (lncRNA) has been found to play an important role in cardiovascular diseases including heart failure, myocardial infarction, and atherosclerosis. The purpose of this study is to identify the expression profile of lncRNA in AF patients, explore the function of lncRNA in AF, and provide a potential scientific basis for the treatment of AF in the future. The “CIBERSORT” R kit was used to analyze 22 immune cell infiltrates in AF and sinus rhythm (SR) patients. GO, KEGG, and GSVA results showed that abnormally expressed lncRNAs were involved in signaling pathways related to the atrium, including the Toll-like receptor signaling pathway and calcium signaling pathway. The physiological functions of these differentially expressed lncRNAs may be related to the pathogenesis of AF, which provide a scientific basis for the prognosis and treatment of patients with AF
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