Identification of apolipoprotein C-I as a potential Wilms' tumor marker after excluding inflammatory factors.

Junjie Zhang,Lei Wang,Jiekai Yu,Da Zhang,Wei Zhao,Heying Yang,Lili Niu,Fei Guo,Jiaxiang Wang,Shu Zheng,Fuquan Yang

doi:10.3390/ijms150916186

Abstract

Wilms’ tumor is one of the most common malignant tumors observed in children, and its early diagnosis is important for late-stage treatment and prognosis. We previously screened and identified protein markers for Wilms’ tumor; however, these markers lacked specificity, and some were associated with inflammation. In the current study, serum samples from children with Wilms’ tumors were compared with those of healthy controls and patients with systemic inflammatory response syndrome (SIRS). After exclusion of factors associated with inflammation, specific protein markers for Wilms’ tumors were identified. After comparing the protein peak values obtained from all three groups, a protein with a m/z of 6438 Da was specified. Purification and identification of the target protein using high-pressure liquid chromatography (HPLC) and two-dimensional liquid chromatography-linearion trap mass spectrometry(2D-LC-LTQ-MS) mass spectrometry, respectively, revealed that it was apolipoprotein C-I (APO C-I). Thus, APO C-I is a specific protein marker for Wilms’ tumor.

Highlights

IntroductionThe major factors influencing the prognosis of children with Wilms’ tumor include early diagnosis, pathological classification, and appropriate treatment, including surgery or chemotherapy for children with stage I and II tumors and tumor resection combined with adjuvant chemotherapy or even radiotherapy for high-stage tumors [2]
Wilms’ tumor is the most common solid malignant abdominal tumor in children [1]
The pretreated sera from patients with Wilms’ tumor, healthy children and children with systemic inflammatory response syndrome (SIRS) were screened with SELDI–TOF–MS to obtain the peak value of related proteins, peak value of inflammatory factors, and their decomposed peptides

Summary

Introduction

The major factors influencing the prognosis of children with Wilms’ tumor include early diagnosis, pathological classification, and appropriate treatment, including surgery or chemotherapy for children with stage I and II tumors and tumor resection combined with adjuvant chemotherapy or even radiotherapy for high-stage tumors [2]. Most patients are diagnosed, late diagnosis delays treatment and may affect prognosis. Tumor markers can enable early diagnosis and permit monitoring therapeutic outcomes [3]. We previously screened and identified specific protein markers for Wilms’ tumor using proteomics analyses of patient sera, the analysis was confounded by the presence of inflammatory factors, which may affect their efficacy for monitoring and diagnosis of Wilms’ tumor. It is necessary to exclude inflammatory factors during screening and identification of tumor markers [4,5]

Methods

Results

Discussion

Conclusion