Abstract
A novel aged garlic extract, with high antioxidant activities, was prepared by soaking sliced garlic in 10% ethanol solution for 90 days. In order to locate the antioxidant ingredients of aged garlic extract, 2,2-azinobis-6-s (3-ethylbenzothiazoline sulfonic acid) assay, hydroxyl free radical assay, 2,2-diphenyl-1-picrylhydrazyl assay, and ferric reducing/antioxidant power assay were conducted to guide the fractionation of aged garlic extract by means of extraction. As a result, the ethyl acetate fraction was identified as the highest antioxidant activity in aged garlic extract. Therefore, the ingredient information of the ethyl acetate fraction were obtained through gas chromatography–mass spectrometry and ultra performance liquid chromatography mass spectrometry / mass spectrometry analysis. Some phenols and diallyl disulphide, 5-(hydroxymethyl)-2-furancarboxaldehyde, S-allyl-cysteine, S-allyl-mercaptocysteine, and 1-methyl-1,2,3,4-tetrahydro-β-carboline-3-carboxylic acid were identified as potent antioxidants in aged garlic extract by gas chromatography–mass spectrometry and ultra performance liquid chromatography mass spectrometry / mass spectrometry, respectively. The biological activities and formation mechanisms of these identified compounds were discussed.
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