Abstract
Outbreaks of enterically transmitted hepatitis involving many thousands of cases have occurred in southern and central Asia, North Africa, and Latin American countries. Hepatitis E virus (HEV), a major cause of the disease is a 27-32nm virus that has features resembling those of "small round structured viruses" (e.g. Norwalk agent). Usually, for identification by electron microscopy, immune electron microscopy (IEM) is required to trap or aggregate the virus.Analysis of the HEV genome has identified 3 open reading frames (ORF) within the positive single-stranded RNA 7.5kb molecule. ORF1 appears to code for nonstructural proteins, while ORF2 and ORF3 are considered to code for structural capsid-associated proteins. Recently, the antigenic nature of the ORF2 and 3 coded putative structural proteins was shown in various immunoassays by the application of synthetic peptides and recombinant proteins. In this study, we tested whether antisera (guinea pig) to immunoreactive synthetic peptides encoded by ORF2 and ORF3 would identify HEV by IEM.
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Schedule a callMore From: Proceedings, annual meeting, Electron Microscopy Society of America
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