Identification of anisakid nematode infection on skipjack (Katsuwonus pelamis L.) from savu sea, east nusa tenggara, Indonesia

Abstract

Nematodes, Anisakis spp, were reported to cause zoonoses in many countries. This parasite commonly infects marine mammals, fish, crustaceans and cephalopods in a wide geographical distribution. Some morphological and molecular studies explained that Anisakis spp.was detected in some marine areas, including Indonesia. This study was aimed to identify the type of nematodes that infect the skipjack tuna (K. pelamis) in Savu Sea. Quantitative description used prevalence studies on the number of fish infected and the average infection intensity, and then we conducted morphological and molecular identification. Morphological analysis was performed on the structure and the characteristic of the larvae. Molecular detection used PCR with ITS (ITS 1, 58s rDNA gene and ITS2), followed by sequencing with the ABI Prisms 3730 XL and the results were analyzed using MEGA 5.03. Taxonomically, the result showed that K. pelamis of Savu Sea, East Nusa Tenggara, Indonesia, was infected by Anisakis typica. * Corresponding Author: L.C. Soewarlan  lady.cindy@ymail.com