Abstract
In the present study, we compared the expression of miRNAs and angiogenesis-related genes in the renal tumors and adjacent normal renal tissues of patients with clear cell renal cell carcinoma (ccRCC). The first part of the present study was a preliminary analysis of 4patients with stage T1a/b ccRCC that measured the levels of angiogenesis and expression of angiogenesis-related genes and miRNAs in the tumors and adjacent normal renal tissues. The second part of this study was an analysis of 30 patients with stage T1, T2 or T3 ccRCC that employed qPCR to characterize expression of angiogenesis-related miRNAs in the tumors and adjacent normal tissues. The first part of this study indicated that all 4 patients had increased levels of CD34 in tumors, indicating elevated angiogenesis. However, quantitative analysis of microvessel density and expression of miRNAs indicated highly variable results among these patients. The data of all patients in the present study indicated that more patients with stageT1 ccRCC had higher expression of miR-126 and miR-378 in their normal tissues, whereas more patients with stage T2/3 ccRCC had higher expression of these miRNAs in their tumor tissues. The tumors of patients with ccRCC had lower expression of miR-126 and miR-378 during the early stages of disease (T1), but higher expression of these miRNAs during the later stages of disease (T2/T3).
Highlights
The incidence of renal cell carcinoma (RCC) has increased steadily over the past 5 decades, and there are well-known demographic and lifestyle factors associated with increased risk [1,2]
Patients with metastatic RCC may be administered chemotherapy with FUDR, 5-FU, Taxol and carboplatin, but the response rate is poor [5] due to the development of multidrug resistance mediated by high expression of P-glycoprotein [6], an ABC-transporter that is responsible for drug efflux
We compared the expression of miRNAs and angiogenesis‐related genes in the renal tumors and adjacent normal renal tissues of patients with Clear cell RCC (ccRCC)
Summary
The incidence of renal cell carcinoma (RCC) has increased steadily over the past 5 decades, and there are well-known demographic and lifestyle factors associated with increased risk [1,2]. Accumulation and increased activity of HIF, and increased expression of VEGF and PDGF, are associated with increased angiogenesis and metastatic potential of RCC [8,9]. Previous research identified miR-210 as a hypoxia-regulated miRNA that was upregulated in RCC [18]. Hauser et al [20] studied RCC patients from Germany and reported that the serum levels of miR-26a-2, miR-191, miR-337-3p and miR-378 did not provide useful diagnostic or prognostic information. They concluded that the level of miR-378 was unrelated to pT-stage, lymph node/ distant metastasis, vascular invasion and tumor grade. We compared the expression of miRNAs and angiogenesis‐related genes in the renal tumors and adjacent normal renal tissues of patients with ccRCC
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